Influence de la présence de la fourmi Wasmannia auropunctata (Roger 1863) (Hymenoptera : Formicidae) sur les autres espèces de fourmis dans la réserve de la Lopé (centre du Gabon)

Résumé

Wasmannia auropunctata (Roger 1863) est une fourmi originaire d’Amérique du Sud. Elle a été introduite au Gabon vers 1920 par des agronomes qui l’ont utilisée comme agent de lutte biologique contre certains insectes parasites du cacaoyer. Aujourd’hui, cette fourmi se retrouve en zone forestière même hors des anciennes plantations de cacaoyers. Depuis 1984, sa présence a été signalée dans le parc national de la Lopé qui fait partie des réserves protégées du Gabon. Des études antérieures réalisées en Nouvelle-Calédonie ont montré que la présence de W. auropunctata a d’énormes conséquences sur la biodiversité car elle a fait disparaître l’ensemble des fourmis de cette région. Quel peut donc être l’impact de sa présence au Gabon dans la zone de la Lopé? Ces études préliminaires de l’impact de W. auropunctata sur la biodiversité de la Lopé ont consisté à évaluer la dispersion de cette espèce et la densité relative des autres espèces de fourmis en présence sur le site. Les résultats montrent que W. auropunctata se répartit selon un gradient décroissant du point d’introduction vers l’intérieur de la forêt jusqu’à une distance de 120 m. Il n’y a pas de W. auropunctata au-delà de cette distance. Du point d’introduction jusqu’à 80 m de distance, la densité relative des autres espèces de fourmis varie de 0 à 10 %. Lorsqu’il y a forte densité de W. auropunctata, les autres espèces de fourmis sont absentes.     

One-step affinity purification of fusion proteins with optimal monodispersity and biological activity: application to aggregation-prone HPV E6 proteins

Background

Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves their folding and solubility, but self-association may still occur. For instance, HPV E6 oncoproteins, when produced as MBP-E6 fusions, are expressed as mixtures of biologically inactive oligomers and active monomers. While a protocol was previously developed to isolate MBP-E6 monomers for structural studies, it allows the purification of only one MBP-E6 construct at the time. Here, we explored a parallelizable strategy more adapted for biophysical assays aiming at comparing different E6 proteins.

Results

In this study, we took advantage of the distinct size and diffusion properties of MBP-E6 monomers and oligomers to separate these two species using a rapid batch preparation protocol on affinity resins. We optimized resin reticulation, contact time and elution method in order to maximize the proportion of monomeric MBP-E6 in the final sample. Analytical size-exclusion chromatography was used to quantify the different protein species after purification. Thus, we developed a rapid, single-step protocol for the parallel purification of highly monomeric MBP-E6 samples. MBP-fused HPV16 E6 samples obtained by this approach were validated by testing the binding to their prototypical peptide targets (the LXXLL motif from ubiquitine ligase E6AP) by BIAcore-SPR assay.

Conclusions

We have designed a rapid single-step batch affinity purification approach to isolate biologically active monomers of MBP-fused E6 proteins. This protocol should be generalizable to isolate the monomer (or the minimal biologically active oligomer) of other proteins prone to self-association.

     

Rapid SERS monitoring of lipid‐peroxidation‐derived protein modifications in cells using photonic crystal fiber sensor

We proposed a side channel photonic crystal fiber (SC‐PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation derived protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC‐PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference‐free alkyne Raman peak from these proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation derived protein modification in cells.

     

Intimate Rendezvous in a Tritrophic Context? Nothing but the Girls for Male Lysiphlebus testaceipes

In insects, mating often occurs after natal dispersal, and hence relies on a coevolved combination of sexual communication and movement allowing mate encounter. Volatile sex pheromones are widespread, generally emitted by females and triggering in‐flight orientation of conspecific males. In parasitoid wasps, unmated females can start laying unfertilized eggs via parthenogenesis so that host patches could serve as sites of rendezvous for mating. Males could therefore use cues associated with host patches to focus their search on females that have successfully found oviposition sites. We hypothesized that in parasitoids exploiting herbivorous hosts, sex pheromones, and herbivore‐induced plant volatiles (HIPV) should act in synergy, triggering male orientation toward ovipositing females. We tested this hypothesis with the aphid parasitoid Lysiphlebus testaceipes. Results from both field and laboratory experiments show that males are strongly attracted to virgin females, but that volatiles from aphid‐infested plants have no effect on male orientation, neither has a cue, nor in interaction with the female sex pheromone. The absence of synergy between sex pheromones and HIPV contrasts with results on other species and raises interesting questions on mating systems and sexual selection in parasitoid wasps.     

Determination of dendrigraft poly-L-lysine diffusion coefficients by taylor dispersion analysis

This work focuses on the physicochemical characterization of dendrigraft poly-L-lysines (DGLs) obtained by polymerization of N-carboxyanhydride in buffered water (pH 6.5). Diffusion coefficients (D) and hydrodynamic radii (Rh) of five successive DGL generations were determined by Taylor dispersion analysis (TDA). To our knowledge, this is the first experimental work using TDA for the characterization of dendrimer-like structures. Experimental Rh values obtained by TDA were compared to those derived from dynamic light scattering and size exclusion chromatography coupled to a triple detection (refractive index, viscosimetry, and static light scattering). Significant differences were obtained, especially for the highest generations, as a result of the inherent contribution of aggregates to the light scattering intensity. For that reason, TDA was found to be the most appropriate technique for determining the D values of these hyperbranched macromolecules. Regarding their physicochemical behavior, the experimental results confirm that DGLs are very similar to trifunctional dendrimers (exponential growth of the molar mass, almost linear variation of the hydrodynamic radius, high branching density, and maximum of the intrinsic viscosity or of the free volume fraction for generation 4).     

m5C Methylation Guides Systemic Transport of Messenger RNA over Graft Junctions in Plants

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Edaphic factors and flower colour distribution in theAnemoneae (Ranunculaceae)

Variation patterns of flower colour in some species of theAnemoneae can be correlated with edaphic variables. These examples are used in the argument that discriminative pollination through colour responsive pollen vectors is not the sole cause for non-randomness in intraspecific flower colour distributions. Some possible reasons for genetic linkage between genes controlling edaphic responses and genes controlling flower pigmentation are discussed.     

Hydrocarbons,sterols and tocopherols in the seeds of six Adansonia species

The composition of the unsaponifiable matter of the lipids of six Adansonia species (A. grandidieri, A. za, A. fony, A. madagascariensis, A. digitata and A. suarezensis) was investigated. The total unsaponifiable content, its general composition and the identity of the components of the hydrocarbon, sterol and tocopherol fractions are presented. The unsaponifiable content in oil ranges from 0.4 to 1.1% (hexane method) and from 0.6 to 2.2% (diethyl ether method). In two species (A. grandidieri and A. suarezensis) the major components are 4-demethylsterols (23–42%) tocopherols (37-10%) and hydrocarbons (15–17%). In both species examined, eight 4-demethylsterols occur in the sterol fraction with sitosterol (81–88%) being predominant. Among the four tocopherols present, γ-tocopherol (68–98%) is the major compound. Each Adansonia species shows a characteristic gas liquid chromatography pattern for the hydrocarbon fraction. Squalene is the major component for five species (40–75%). Iso-, anteiso- and other branched hydrocarbons were not identified but were present in small amounts in comparison with n-alkanes. The dominance of odd- over even-carbon number chain length of n-alkanes was not observed in any species. The results show that C₂₂, C₂₅, C₂₆, C₂₇, C₂₈ and C₂₉ are the most frequent major constituents.     

Synthesis,crystal structures and properties of three new lanthanide 2,6-pyridinedicarboxylate complexes with zero-dimensional structure

Three new lanthanide dipicolinate complexes with zero-dimensional structure have been synthesized by mild hydrothermal method. In these complexes the lanthanide is surrounded by three dipicolinate groups in the usual tridentate mode. For two of them, (CN₃H₆)₃[La(C₇H₃NO₄)₃] · 3H₂O I and (C₄N₂H₁₂)_1.5[Ce(C₇H₃NO₄)₃] · 7H₂O II, the dipicolinate groups are wholly unprotonated. The resulting molecular entity is three-times negatively charged and the balance charge is insured by the presence of guanidinium or piperazinium ions for the first and second, respectively. For the third complex, [Eu(C₇H₄NO₄)₃]₂ · 2.5H₂O III, one hydrogen atom remains globally on each dipicolinate and so the molecular entities are neutral. For the latter complex, the resulting molecular entities are connected into chains through strong hydrogen bonding. Thermogravimetric analyses and luminescent behaviour of complex III have been carried out.     

Dynamic Co-evolution of Peptides and Chemical Energetics, a Gateway to the Emergence of Homochirality and the Catalytic Activity of Peptides

We propose a scenario for the dynamic co-evolution of peptides and energy on the primitive Earth. From a multi component system consisting of hydrogen cyanide, several carbonyl compounds, ammonia, alkyl amine, carbonic anhydride, borate and isocyanic acid, we show that the reversibility of this system leads to several intermediate nitriles, that irreversibly evolve to alpha-amino acids and N-carbamoyl amino acids via selective catalytic processes. On the primitive Earth these N-carbamoyl amino acids combined with energetic molecules (NOx) may have been the core of a molecular engine producing peptides permanently and assuring their recycling and evolution. We present this molecular engine, a production example, and its various selectivities. The perspectives for such a dynamic approach to the emergence of peptides are evoked in the conclusion.